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ARPN Journal of Agricultural and Biological Science                             January 2018 | Vol. 13  No. 1

Title: A study on molecular surveillance of theileria spp. infection and its impact on hematological and biochemical changes in naturally infected small ruminants at Multan, Pakistan Author (s): Amir Sohail, Muhammad Yasir Khan, Tanvir Burni, Farhatullah, Iftikhar Hussain Khalil and Syed Mehar Ali Shah Abstract: The current investigation was conducted to evaluate hematological and biochemical changes in naturally infected sheep and goats with Theileria spp. infection and to compare the conventional microscopic method of diagnosis with PCR amplification. A total of 200 blood samples (144 sheep and 56 goats) were collected and examined for the presence of Theileria infection. 12.5% and 39.5% blood samples were found positive with Theileriainfection by microscopic examination and PCR amplification respectively. Hematological parameters were analyzed by automatic hematology analyzer (Diatron Abacus) while serum biochemistry was studied by Semi-automatic Chemistry Analyzer (Micro Lab 300). Hematological parameters revealed significant (p < 0.05) decrease in RBCs counts, Hb contents, PCV, MCH and MCHC values while non-significant (p > 0.05) correlation in MCV values of Theileria infected small ruminants. Biochemical analysis revealed significant (p < 0.05) reduction in total serum protein and albumin concentration while non-significant (p > 0.05) rise in urea and cholesterol level in infected animals compared to normal. Theileriapiroplasms prevalence during early stage of disease without any clinical symptoms can only be identified through PCR amplification compared to microscopic examination.             Full Text   Title: Hypolipidimicand antioxidant activities of cold and hot-pressed linseed oil in high-cholesterol fed rats Author (s): Bandar H. Aloufi Abstract: Hyperlipidemia is a metabolic disorder of plasma lipids, triglycerides, cholesterol, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C). Hyperlipidemia is among the leading risk factors associated with cardiovascular diseases. This study aimed to compare the efficiency of cold and hot-pressed linseed oil to decrease the levels of total cholesterol and proteins in high cholesterol fed- Wister rats (n =60) weighing (225-252 g). Linseed oil was fed for seven weeks to Wister rats and control. Many biochemical and physiological parameters were measured by automatic analyzer and the results were statistically analyzed by SPSS.12. In comparison to hypercholesterolemic diet- control rats showed highly significant decrease in values of following physiological parameters; glucose, triglycerides, total cholesterol, low density lipoprotein cholesterol, total protein. In contrast, marked increasing in the value of high density lipoprotein cholesterol when compared with hypercholesterolemic diet- control rats. Highly significant decrease in rats fed with hypercholesterolemic diet enriched with (5g/100g cold pressed linseed oil), in blood measurements of triglycerides, total cholesterol, and low density lipoprotein cholesterol. In contrast, marked increasing in the value of high density lipoprotein cholesterol when compared with rats fed with hypercholesterolemic diet enriched with (5g/100g hot pressed linseed oil). These findings indicate that diets containing Linseed oil significantly improved the physiological parameters of rats. We suggest that Linseed oil as part of food might improve blood parameters and increase high density lipoprotein cholesterol in rats. We further suggest that Linseed oil supplementation act as antioxidant agents, and an excellent adjuvant therapy for rats.       Full Text