ARPN Journal of Agricultural and Biological Science       ISSN 1990-6145
   
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ARPN Journal of Agricultural and Biological Science                               June 2017 | Vol. 12  No. 6
 
Title:

Characterization and controlling the specification of definitive hematopoiesis during the development of zebrafish

Author (s):

Nibras Najm Abbood and Amir Abdullah Jabir

Abstract:

Modern studies propose that, in the primary mesoderm, there is collaboration between the hematopoietic and cardiac lineages. Though, whether miRNAs can affect other lineages remains unidentified. Consequently, we have investigated whether hematopoietic miR-142-3p modulated the mesoderm formation. We account that knockdown of miR-142-3p, a hematopoietic-specific miRNA, in zebrafish occasioned in damage of hematopoiesis during embryonic development. Interestingly, we detected abnormal cardiac phenotypes and inadequacy of somitegenesis in KD-morphants. In the early developmental phase, a tiny heart, contractile dysfunction in the ventricle, cardiac arrhythmia and bradycardia were constantly observed. Histological examination exposed unadorned hypoplasia of the ventricle and disrupted muscle alignment. To determine the mechanism, we executed DNA microarray analysis. The results exposed that the expression of several mesodermal genes necessary for the development of cardiac and somatic mesoderm, such as no tail, T-box gene 16, mesoderm posterior a, one eye pinhead, and rho-associated, coiled-coil containing protein kinase (Rock2a), were augmented in miR-142-3p KD-morphants. In this paper, the characterization and controlling the specification of definitive hematopoiesis during the development of zebrafish has been elaborated.

 
       
 
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Title:

Antioxidants content of olive oil produced from two olive varieties at differentharvesting stages

Author (s):

Radwan Y. Ajo, Yousef H. Tawalbeh, YahyaAL-Qudah, Walid M. Al-Rousan, Nazieh A. Al Khalaileh and Baker M. Albow

Abstract:

The objective of this study was theinvestigation of the antioxidant content of olive oil from two varieties Nabali and Rumiat different harvesting stages in order to achieve the optimum harvesting date. The proper harvesting dates were determined using maturation index according to International Olive Council method. Our results showed that the proper harvesting stage for Rumi olives was during the periods October 24thtoOctober 28th, whereas, the proper harvesting stage for Nabali olives was between November 4thandNovember 7th. The acidity and peroxide value of olive oil from two varieties was within the range of extra virgin olive oil according to IOC. The results showed a significant decrease in squalene contents in Rumi and Nabali olive oil parallelly with the progress of the harvesting stages. In general, most of antioxidants contents of olive oil of the two selected olive varieties were gradually increase up to the proper harvesting dates then declined.

 
 
 
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Title:

Investigating the variation of morphological and physiological traits of native pear genotypes of Sardasht city

Author (s):

Fereidoon Alipoor

Abstract:

The pear, after apple, considered as the most important grainy fruits of the world and Iran. According to findings of researchers the west of Iran is part of the center of origin pear fruit and the West Azerbaijan province, according to the topographical status and geographical conditions, its vegetation has great diversity. Native Pear in this province, especially in the highlands and jungles of Sardasht city as types of wild pear has a great variety. Accordingly, investigating of the morphological and physiological traits of native pear of Sardasht based on national guideline tests for distinctness, uniformity, and stability (DUS (was conducted. There were, respectively 5and 9 traits of the shape of trees and one-year branch, among morphological traits and among physiological traits which 15 traits related to fruit, were investigated. This research, all genotype kinds of native pear, after identifying the trees and coding them was done in two stages and the considered traits were written and in a stage in the laboratory the chemical traits studied and statistical investigations were done using SPSS software. Chart analysis and resulted cluster analysis showed the number of 8 types of native pear genotype and showed its close relative value. In general, according to variance analysis of biochemical traits, the correlation was observed between all traits that most of the trees had abundant diversity. The collection of key traits was, well able to make differentiation and distinction between various native pear trees of Sardasht area.

 
 
 
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