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          | ARPN Journal of Agricultural and Biological 
          Science         
          November 2009 | Vol. 4  No. 6 |  
      
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                    | Title: | Submerged 
                    liquid fermentation of some Tanzanian basidiomycetes for the 
                    production of mycelial biomass, exopolysaccharides and 
                    mycelium protein using wastes peels media
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                    | Author (s): | Anthony Manoni Mshandete 
                    and Jehova R. Mgonja
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                    | Abstract: | 
                    Mycelia from five species 
                    of higher fungi of genera Ganoderma, Pleurotus and 
                    Laetiporus were used in this study. These were cultured 
                    for the investigation of higher exopolysaccharides and 
                    mycelia biomass production. Mycelial growths of all species 
                    were measured on six different non-defined complex media 
                    prepared from waste peels and cattail rhizome; Yam dextrose 
                    agar (YADA), cassava dextrose agar (CADA), potato malt 
                    extract peptone dextrose agar (PMPDA), sweet potato dextrose 
                    agar (SPDA), plantain dextrose agar (PLDA) and cattail 
                    rhizome dextrose agar (CATDA). The average mycelial growth 
                    rate on the aforementioned solid media ranged between 10 ± 
                    2.6 and 14 ±1.7 mm/day with the highest growth rate obtained 
                    from YADA followed by PMPDA while the lowest was recorded 
                    from PLDA. The best media based on mycelial growth were 
                    selected for further investigation. Therefore, the effect of 
                    YAD and PMPD complex media on the submerged mycelia growth 
                    and exopolysaccharides, mycelium protein production in five 
                    mushrooms was investigated in shake-flask culture at an 
                    ambient temperature 30oC±2. The maximum mycelial growth 
                    (14g/l) and exopolysaccharides production (540mg/l) were 
                    achieved in YAD medium by Pleurotus spp and 
                    Pleurotus HK-37, respectively. The crude protein content 
                    of mushroom mycelium in YAD medium varied among the mushroom 
                    species. The Pleurotus spp mycelium contained 55% 
                    crude protein, which was the highest followed by 41% 
                    obtained from Pleurotus HK-37 mycelium. The genus 
                    Pleurotus amongst the other mushroom genera 
                    investigated, should be considered for reasonable production 
                    of mycelial biomass, exopolysaccharides and mycelium protein 
                    using YAD medium in submerged fermentation. In conclusion, 
                    the data obtained in this study provides useful information 
                    for further investigation of higher fungi in submerged 
                    culture using waste peels complex media.
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                    | Title: | Kinetics of 
                    water sorption by egusi melon (Cucumeropsis edulis) seeds |  
                    | Author (s): | A. Addo and A. 
                    Bart-Plange |  
                    | Abstract: | 
                    The kinetics of water 
                    absorption by egusi melon seeds was studied by the 
                    gravimetric method during soaking for a temperature range of 
                    30-70°C to determine its moisture diffusivity. The water 
                    diffusion coefficient of the grain was in the range 5.18x10-8 
                    to 20.99x10-8 m2/s. An Arrhenius-type 
                    equation described the strong temperature effect on the 
                    diffusion coefficient with activation energy of 28.38 
                    kJ/mol. It was shown that a satisfactory prediction of water 
                    absorption during soaking of the egusi was possible by 
                    fitting experimental data to Becker’s model. |  
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                    | Title: | Biowaste 
                    materials as sorbents to remove chromium(vi) from aqueous 
                    environment- A comparative study |  
                    | Author (s): | V. Vinodhini 
                    and Nilanjana Das |  
                    | Abstract: | 
                    The adsorption of chromium (VI) ions from 
                    aqueous solutions has been investigated on Neem sawdust 
                    (NS), Mango sawdust (MS), Wheat shell (WS), Sugarcane 
                    bagasse (SB) and Orange peel (OP). The adsorbent which 
                    showed highest chromium(VI) removal was neem sawdust. The 
                    influence of pH, contact time, biomass dosage and initial 
                    metal concentration on biosorption was investigated. The 
                    biosorptive capacities of the biosorbents were dependent on 
                    the pH of the chromium solution, with pH 2 being optimal. 
                    The adsorption data fitted well with the Langmuir isotherm 
                    model. The adsorption capacities were found to be 58.82, 
                    37.73, 28.08, 23.8 and 23.3 mg/g for NS, MS, WH, SB and OP, 
                    respectively.  |  
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                    | Title: | Qualitative 
                    analysis of serum proteins in benign prostatic hyperplasia 
                    separated by SDS-PAGE |  
                    | Author (s): | Saima Naz, 
                    Sarah Ahmad and Farkhanda Ghafoor |  
                    | Abstract: | 
                    This study was conducted for qualitative 
                    analysis of serum proteins separated by SDS-PAGE and stained 
                    by Coomassie Brilliant Blue R-250 in order to describe the 
                    preliminary identification of serum proteins that may act as 
                    diagnostic marker in benign prostatic hyperplasia. Serum 
                    samples of 23 biopsy confirmed cases of benign prostatic 
                    hyperplasia and normal controls of similar age group were 
                    subjected to SDS-PAGE on a 12% resolving gel, followed by 
                    staining with Coomassie Brilliant Blue R-250. Protein 
                    fractions were analyzed using computer software program 
                    “GeneGenius Gel Documentation and Analysis System”. Major 
                    protein fractions ranging in molecular weights from 1.45-157 
                    kDa were observed. Raw volumes of most of the protein 
                    fractions seem to be increased in majority of benign 
                    prostatic hyperplasia cases as compared to normal control. 
                    Protein fractions 55-57 kDa were undetected in normal 
                    controls under 80 years of age but appeared in 56% of benign 
                    prostatic hyperplasia cases. Two dimensional gel 
                    electrophoresis and silver staining of these samples could 
                    yield better resolution of protein fraction 55-57 kDa that 
                    could serve as marker for benign prostatic hyperplasia. |  
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                    | Title: | Influence of 
                    paddy husk on the ripening of fruit of Zizyphus 
                    mauritiana Lamk |  
                    | Author (s): | A. Ezhilarasi 
                    and C. Tamilmani |  
                    | Abstract: | 
                    The present investigation was aimed to study 
                    the influence of paddy husk on the ripening of detached 
                    fruits of Zizhyphus mauritiana Lamk. The control 
                    fruits were kept in the laboratory (Room Temperature), while 
                    the experimental fruits were treated with paddy husk. The 
                    fruits retained their green colour only for two days, on the 
                    third day the fruit colour changed to greenish yellow. While 
                    on the fourth day the colour become brownish. Hence, the 
                    acceptable storage period of  Zizhyphus mauritiana 
                    fruits is only four days and afterwards the fruits became 
                    over ripened. All the studies were carried out using the 
                    peel and pulp of the fruit tissues individually and the 
                    following results were obtained during the process of 
                    ripening. The Fruit firmness, Titratable acidity, 
                    chlorophyll content, proteins, starch, ascorbic acid and 
                    phenols decreased during ripening both in the treated and 
                    control fruits. On the other hand, total soluble solids, pH, 
                    carotenoids, anthocyanin and sugar increased. |  
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                    | Title: | A study to 
                    reduce salt usage in preservation of skins and hides with 
                    alternate use of plant extract |  
                    | Author (s): | V. Sivabalana 
                    and A. Jayanthib |  
                    | Abstract: | 
                    Raw hides and skin are preserved before 
                    processed into leather. Salt preservation is the general and 
                    age old popular practice. Possibilities of using other 
                    methods of preservations have been analyzed in order to 
                    reduce the pollution load in the soaking process of leather 
                    by many scientist. However in the present study less salt 
                    preserving systems based on herbal extract have been 
                    investigated and evaluated at ambient temperature of 30-37şC 
                    as alterative preservation system. The efficacy of this 
                    alternative system was assessed by parameters such as 
                    determination of bacterial count, isolation and 
                    characterization of microorganism from raw goat skin, 
                    antimicrobial activity by using herbal extraction with well 
                    diffusion method, percentage weight loss during storage of 
                    cured skin, determination of hydrothermal stability of the 
                    skin and physical properties of finished leather was 
                    conducted. 12 plants were screened for antimicrobial assay 
                    among which only 5 plants had antimicrobial activity 
                    Weddilia chininsis, Cassia alatta, 
                    Clerodentron pholomides, Solanum trilobotum, 
                    Calotropis procera. Hence these 5 plants where chosen 
                    for direct application on the on skin along with reduced 
                    percentage of NaCl. |  
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                    | Title: | Proline level 
                    and amylase and ascorbate peroxidase activity in the 
                    germination of Plantago ovata forsk (plantaginaceae) 
                    seeds |  
                    | Author (s): | Lúcia F. Braga, 
                    Marcilio P. Sousa, Leonardo C. Ferreira, Maria Elena Ap. 
                    Delachiave, Ana C. Cataneo and Joăo F. Braga |  
                    | Abstract: | 
                    The species Plantago ovata has great 
                    importance for the pharmaceutical industry due to the high 
                    mucilage level in its seeds. Thus, the present study aimed 
                    to verify proline level and amylase and ascorbate peroxidase 
                    activity in the germination of seeds subjected to stress, 
                    which was induced by using PEG, NaCl and CaCl2 solutions. 
                    Fifty seeds were placed into filter paper moistened with 15 
                    mL of such solutions at the potentials 0,-0.2, -0.4, -0.6 
                    and -0.8 MPa in the presence of light at 25şC. Proline 
                    levels decreased during Plantago ovata germination 
                    under water and salt stress, whereas the activity of 
                    ascorbate peroxidase and amylase was higher under 
                    PEG-induced stress, relative to those induced by NaCl and 
                    CaCl2. |  
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                    | Title: | Study on root 
                    stimulation of clonal durian rootstock propagation 
                    preliminary results |  
                    | Author (s): | Panca J. 
                    Santoso and Fitriana Nasution |  
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                    Abstract: | 
                    Root stimulation technique on clonal durian 
                    rootstock propagation was evaluated in an experiment 
                    conducted from January 2006 to April 2007 at Indonesian 
                    Tropical Fruit Research Institute, Solok, west Sumatra. The 
                    experiment was arranged in factorial randomized block design 
                    with 4 replications and 35 plants per treatment unit. Two 
                    treatments applied were steam wound techniques: partly 
                    steam-sliced, bark-ringed, rounded skin-removed; and root 
                    growth regulator. After treatment, the stem was then mounted 
                    using media from container surface to 3 cm above the 
                    treatment point. Parameters of plant growth and rooting 
                    characteristic were collected at 4 month after treatment. 
                    Based on the results of observation, it was found that stem 
                    wounding technique has significantly effect to percentage of 
                    plant life, plant height, leaf number, and stem diameter. 
                    Application of root growth regulator was found to have 
                    significantly effect to leaf number, have not significantly 
                    effect to plant height and stem diameter, and have 
                    negatively effect to plant life. All treatments were found 
                    to have not significantly effect to all rooting parameters, 
                    whilst root oppositely grows below the treatment area. It 
                    is, therefore, suggested to narrowing the stimulation area 
                    as that in air layering technique to lead hormone 
                    accumulation on the treatment area. |  
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                    | Title: | Genetic 
                    variation for yield and yield components in rice |  
                    | Author (s): | Jamal, 
                    Ifftikhar H. Khalil, Abdul Bari, Sajid Khan and Islam Zada |  
                    | Abstract: | 
                    Five exotic rice genotypes along with a local 
                    check were evaluated for yield and yield contributing traits 
                    under the climatic conditions of Swat.  Minimum days to 
                    flowering were taken by rice genotype YUNLEN2 (59.7 days) 
                    and were 14 days early in maturity than check. Minimum Flag 
                    Leaf Area of 10 cm2 was taken by rice genotype 
                    PR26881-JP16-4B-78-5-1 and the maximum for IRI 384 (13.1cm2). 
                    Minimum numbers of tillers plant-1 of 10.7 were 
                    taken by rice genotype IRI384. The PR26881-JP16-4B-78-5-1 
                    took maximum number of 15.3 tillers plant-1. Minimum plant 
                    height of 82.2 was recorded by YUNLEN2 while maximum heights 
                    of 124.9 were recorded by J.P.5. Minimum panicle length was 
                    recorded by IRI384 i.e. 19.2, while maximum panicle lengths 
                    of 24.1 were recorded by J.P.5. Minimum primary branches 
                    paninle-1 of 9.3 was recorded by IRI 384, while maximum 
                    primary branches panicle-1 of 11.3 were recorded 
                    by J.P.5. Minimum number of grain panicle-1 of 10 
                    was recorded by PR26881-JP16-4B-78-5-1, while maximum 
                    numbers of grain panicle-1 of 13.1 were recorded 
                    by IRI384. Minimum 1000-grain weight was recorded by IRI384, 
                    while maximum 1000-grain weights were recorded by ILLABONG. 
                    Minimum Grain yield plant-1 was recorded by 
                    YUNLEN2, while maximum grain yield plant-1 was 
                    recorded by J.P.5.  |  
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                    | Title: | Effect of 
                    juvenile hormone mimic r394 on silkworm (Bombyx mori 
                    L.) growth and development of silk gland |  
                    | Author (s): | S. K. Gangwar |  
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                    Abstract: | 
                    The effect of a juvenile hormone (JH) mimic 
                    R394 (ethyl 9-cyclohexyl-3, 7-dimethy l-2, 4-nonadienoate) 
                    applied topically on the abdominal tergum of silkworm (Bombyx 
                    mori L.) after the fourth ecdysis has been studied. JH 
                    R394 ranging from 1.0, 0.1, 0.01 µl / larva was applied at 
                    48, 72 and 96 hours of V instar larva that was improved the 
                    cocoon weight, shell weight and shell ratio etc. The most 
                    effective dose observed 0.01 µl / larva, when applied at 48 
                    and 72 hours after resumption of the last moult. It was 
                    observed that the development of the silk gland takes place 
                    up to a particular period, after that the larval development 
                    increases due to the increase of the feeding period without 
                    any significant increase in silk and silk ratio. |  
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