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ARPN Journal of Agricultural and Biological
Science
November 2009 | Vol. 4 No. 6 |
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Title: |
Submerged
liquid fermentation of some Tanzanian basidiomycetes for the
production of mycelial biomass, exopolysaccharides and
mycelium protein using wastes peels media
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Author (s): |
Anthony Manoni Mshandete
and Jehova R. Mgonja
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Abstract: |
Mycelia from five species
of higher fungi of genera Ganoderma, Pleurotus and
Laetiporus were used in this study. These were cultured
for the investigation of higher exopolysaccharides and
mycelia biomass production. Mycelial growths of all species
were measured on six different non-defined complex media
prepared from waste peels and cattail rhizome; Yam dextrose
agar (YADA), cassava dextrose agar (CADA), potato malt
extract peptone dextrose agar (PMPDA), sweet potato dextrose
agar (SPDA), plantain dextrose agar (PLDA) and cattail
rhizome dextrose agar (CATDA). The average mycelial growth
rate on the aforementioned solid media ranged between 10 ±
2.6 and 14 ±1.7 mm/day with the highest growth rate obtained
from YADA followed by PMPDA while the lowest was recorded
from PLDA. The best media based on mycelial growth were
selected for further investigation. Therefore, the effect of
YAD and PMPD complex media on the submerged mycelia growth
and exopolysaccharides, mycelium protein production in five
mushrooms was investigated in shake-flask culture at an
ambient temperature 30oC±2. The maximum mycelial growth
(14g/l) and exopolysaccharides production (540mg/l) were
achieved in YAD medium by Pleurotus spp and
Pleurotus HK-37, respectively. The crude protein content
of mushroom mycelium in YAD medium varied among the mushroom
species. The Pleurotus spp mycelium contained 55%
crude protein, which was the highest followed by 41%
obtained from Pleurotus HK-37 mycelium. The genus
Pleurotus amongst the other mushroom genera
investigated, should be considered for reasonable production
of mycelial biomass, exopolysaccharides and mycelium protein
using YAD medium in submerged fermentation. In conclusion,
the data obtained in this study provides useful information
for further investigation of higher fungi in submerged
culture using waste peels complex media.
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Title: |
Kinetics of
water sorption by egusi melon (Cucumeropsis edulis) seeds |
Author (s): |
A. Addo and A.
Bart-Plange |
Abstract: |
The kinetics of water
absorption by egusi melon seeds was studied by the
gravimetric method during soaking for a temperature range of
30-70°C to determine its moisture diffusivity. The water
diffusion coefficient of the grain was in the range 5.18x10-8
to 20.99x10-8 m2/s. An Arrhenius-type
equation described the strong temperature effect on the
diffusion coefficient with activation energy of 28.38
kJ/mol. It was shown that a satisfactory prediction of water
absorption during soaking of the egusi was possible by
fitting experimental data to Becker’s model. |
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Title: |
Biowaste
materials as sorbents to remove chromium(vi) from aqueous
environment- A comparative study |
Author (s): |
V. Vinodhini
and Nilanjana Das |
Abstract: |
The adsorption of chromium (VI) ions from
aqueous solutions has been investigated on Neem sawdust
(NS), Mango sawdust (MS), Wheat shell (WS), Sugarcane
bagasse (SB) and Orange peel (OP). The adsorbent which
showed highest chromium(VI) removal was neem sawdust. The
influence of pH, contact time, biomass dosage and initial
metal concentration on biosorption was investigated. The
biosorptive capacities of the biosorbents were dependent on
the pH of the chromium solution, with pH 2 being optimal.
The adsorption data fitted well with the Langmuir isotherm
model. The adsorption capacities were found to be 58.82,
37.73, 28.08, 23.8 and 23.3 mg/g for NS, MS, WH, SB and OP,
respectively. |
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Title: |
Qualitative
analysis of serum proteins in benign prostatic hyperplasia
separated by SDS-PAGE |
Author (s): |
Saima Naz,
Sarah Ahmad and Farkhanda Ghafoor |
Abstract: |
This study was conducted for qualitative
analysis of serum proteins separated by SDS-PAGE and stained
by Coomassie Brilliant Blue R-250 in order to describe the
preliminary identification of serum proteins that may act as
diagnostic marker in benign prostatic hyperplasia. Serum
samples of 23 biopsy confirmed cases of benign prostatic
hyperplasia and normal controls of similar age group were
subjected to SDS-PAGE on a 12% resolving gel, followed by
staining with Coomassie Brilliant Blue R-250. Protein
fractions were analyzed using computer software program
“GeneGenius Gel Documentation and Analysis System”. Major
protein fractions ranging in molecular weights from 1.45-157
kDa were observed. Raw volumes of most of the protein
fractions seem to be increased in majority of benign
prostatic hyperplasia cases as compared to normal control.
Protein fractions 55-57 kDa were undetected in normal
controls under 80 years of age but appeared in 56% of benign
prostatic hyperplasia cases. Two dimensional gel
electrophoresis and silver staining of these samples could
yield better resolution of protein fraction 55-57 kDa that
could serve as marker for benign prostatic hyperplasia. |
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Title: |
Influence of
paddy husk on the ripening of fruit of Zizyphus
mauritiana Lamk |
Author (s): |
A. Ezhilarasi
and C. Tamilmani |
Abstract: |
The present investigation was aimed to study
the influence of paddy husk on the ripening of detached
fruits of Zizhyphus mauritiana Lamk. The control
fruits were kept in the laboratory (Room Temperature), while
the experimental fruits were treated with paddy husk. The
fruits retained their green colour only for two days, on the
third day the fruit colour changed to greenish yellow. While
on the fourth day the colour become brownish. Hence, the
acceptable storage period of Zizhyphus mauritiana
fruits is only four days and afterwards the fruits became
over ripened. All the studies were carried out using the
peel and pulp of the fruit tissues individually and the
following results were obtained during the process of
ripening. The Fruit firmness, Titratable acidity,
chlorophyll content, proteins, starch, ascorbic acid and
phenols decreased during ripening both in the treated and
control fruits. On the other hand, total soluble solids, pH,
carotenoids, anthocyanin and sugar increased. |
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Title: |
A study to
reduce salt usage in preservation of skins and hides with
alternate use of plant extract |
Author (s): |
V. Sivabalana
and A. Jayanthib |
Abstract: |
Raw hides and skin are preserved before
processed into leather. Salt preservation is the general and
age old popular practice. Possibilities of using other
methods of preservations have been analyzed in order to
reduce the pollution load in the soaking process of leather
by many scientist. However in the present study less salt
preserving systems based on herbal extract have been
investigated and evaluated at ambient temperature of 30-37şC
as alterative preservation system. The efficacy of this
alternative system was assessed by parameters such as
determination of bacterial count, isolation and
characterization of microorganism from raw goat skin,
antimicrobial activity by using herbal extraction with well
diffusion method, percentage weight loss during storage of
cured skin, determination of hydrothermal stability of the
skin and physical properties of finished leather was
conducted. 12 plants were screened for antimicrobial assay
among which only 5 plants had antimicrobial activity
Weddilia chininsis, Cassia alatta,
Clerodentron pholomides, Solanum trilobotum,
Calotropis procera. Hence these 5 plants where chosen
for direct application on the on skin along with reduced
percentage of NaCl. |
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Title: |
Proline level
and amylase and ascorbate peroxidase activity in the
germination of Plantago ovata forsk (plantaginaceae)
seeds |
Author (s): |
Lúcia F. Braga,
Marcilio P. Sousa, Leonardo C. Ferreira, Maria Elena Ap.
Delachiave, Ana C. Cataneo and Joăo F. Braga |
Abstract: |
The species Plantago ovata has great
importance for the pharmaceutical industry due to the high
mucilage level in its seeds. Thus, the present study aimed
to verify proline level and amylase and ascorbate peroxidase
activity in the germination of seeds subjected to stress,
which was induced by using PEG, NaCl and CaCl2 solutions.
Fifty seeds were placed into filter paper moistened with 15
mL of such solutions at the potentials 0,-0.2, -0.4, -0.6
and -0.8 MPa in the presence of light at 25şC. Proline
levels decreased during Plantago ovata germination
under water and salt stress, whereas the activity of
ascorbate peroxidase and amylase was higher under
PEG-induced stress, relative to those induced by NaCl and
CaCl2. |
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Title: |
Study on root
stimulation of clonal durian rootstock propagation
preliminary results |
Author (s): |
Panca J.
Santoso and Fitriana Nasution |
Abstract: |
Root stimulation technique on clonal durian
rootstock propagation was evaluated in an experiment
conducted from January 2006 to April 2007 at Indonesian
Tropical Fruit Research Institute, Solok, west Sumatra. The
experiment was arranged in factorial randomized block design
with 4 replications and 35 plants per treatment unit. Two
treatments applied were steam wound techniques: partly
steam-sliced, bark-ringed, rounded skin-removed; and root
growth regulator. After treatment, the stem was then mounted
using media from container surface to 3 cm above the
treatment point. Parameters of plant growth and rooting
characteristic were collected at 4 month after treatment.
Based on the results of observation, it was found that stem
wounding technique has significantly effect to percentage of
plant life, plant height, leaf number, and stem diameter.
Application of root growth regulator was found to have
significantly effect to leaf number, have not significantly
effect to plant height and stem diameter, and have
negatively effect to plant life. All treatments were found
to have not significantly effect to all rooting parameters,
whilst root oppositely grows below the treatment area. It
is, therefore, suggested to narrowing the stimulation area
as that in air layering technique to lead hormone
accumulation on the treatment area. |
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Title: |
Genetic
variation for yield and yield components in rice |
Author (s): |
Jamal,
Ifftikhar H. Khalil, Abdul Bari, Sajid Khan and Islam Zada |
Abstract: |
Five exotic rice genotypes along with a local
check were evaluated for yield and yield contributing traits
under the climatic conditions of Swat. Minimum days to
flowering were taken by rice genotype YUNLEN2 (59.7 days)
and were 14 days early in maturity than check. Minimum Flag
Leaf Area of 10 cm2 was taken by rice genotype
PR26881-JP16-4B-78-5-1 and the maximum for IRI 384 (13.1cm2).
Minimum numbers of tillers plant-1 of 10.7 were
taken by rice genotype IRI384. The PR26881-JP16-4B-78-5-1
took maximum number of 15.3 tillers plant-1. Minimum plant
height of 82.2 was recorded by YUNLEN2 while maximum heights
of 124.9 were recorded by J.P.5. Minimum panicle length was
recorded by IRI384 i.e. 19.2, while maximum panicle lengths
of 24.1 were recorded by J.P.5. Minimum primary branches
paninle-1 of 9.3 was recorded by IRI 384, while maximum
primary branches panicle-1 of 11.3 were recorded
by J.P.5. Minimum number of grain panicle-1 of 10
was recorded by PR26881-JP16-4B-78-5-1, while maximum
numbers of grain panicle-1 of 13.1 were recorded
by IRI384. Minimum 1000-grain weight was recorded by IRI384,
while maximum 1000-grain weights were recorded by ILLABONG.
Minimum Grain yield plant-1 was recorded by
YUNLEN2, while maximum grain yield plant-1 was
recorded by J.P.5. |
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Title: |
Effect of
juvenile hormone mimic r394 on silkworm (Bombyx mori
L.) growth and development of silk gland |
Author (s): |
S. K. Gangwar |
Abstract: |
The effect of a juvenile hormone (JH) mimic
R394 (ethyl 9-cyclohexyl-3, 7-dimethy l-2, 4-nonadienoate)
applied topically on the abdominal tergum of silkworm (Bombyx
mori L.) after the fourth ecdysis has been studied. JH
R394 ranging from 1.0, 0.1, 0.01 µl / larva was applied at
48, 72 and 96 hours of V instar larva that was improved the
cocoon weight, shell weight and shell ratio etc. The most
effective dose observed 0.01 µl / larva, when applied at 48
and 72 hours after resumption of the last moult. It was
observed that the development of the silk gland takes place
up to a particular period, after that the larval development
increases due to the increase of the feeding period without
any significant increase in silk and silk ratio. |
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