Bioactive peptide prediction of skipjack tuna (Katsuwonus Pelamis) hydrolysates using in silico
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Author |
Putut H. Riyadi, Eko Susanto, Tri W. Agustini, Muhammad H. Arifin and Dwi Y. B. Prasetyo
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e-ISSN |
1819-6608 |
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On Pages
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1624-1633
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Volume No. |
18
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Issue No. |
14
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Issue Date |
September 30, 2023
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DOI |
https://doi.org/10.59018/0723202
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Keywords |
katsuwonus pelamis, hydrolysate, peptide, in silico.
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Abstract
Katsuwonus pelamis or locally called skipjack tuna has been used as food for centuries by different populations. It is one of the important sources of protein for human health. Hydrolysate is one of the ways to utilize the primary function of protein as a natural protein source. The hydrolysate is known to have many bioactivates that may benefit human health such as immunodulators, anticancer, antihypertensive, antioxidant and anti-inflammatory. The current study aimed to explore the potential of Katsuwonus pelamis hydrolysate as a potential bioactivity agent. Gene bank of Katsuwonus pelamis obtained from NCBI then analyzed using BIOPEP. The software can predict the potential biopeptides as a bioactive agent. Furthermore, the BIOPEP also capable of predicting the contributing sensory taste based on peptides from Katsuwonus pelamis. The toxicity of promising peptides is then analyzed using ProTox II to determine the exact LD50 of each potential peptide. Results show that the most dominant bioactivities of Katsuwonus pelamis is ACE inhibitor followed by DPP IV inhibitor. Sensory analysis shows that the most contributing taste on Katsuwonus pelamis peptides is bitter followed by sour taste. The potential peptides are dominated by class V toxicity levels with LD50 value of 2000 < LD50 ≤ 5000 mg/kg. The research suggests that Katsuwonus pelamis hydrolysates are proven to be a potential bioactive agent that can be utilized as a functional food for human health benefits.
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